![]() ![]() Western blot results list levels of IgM and IgG antibodies.This test may be less effective in the early going-since antibodies may not have formed-but is very sensitive. Measures are broken down into numbers, with results greater than or equal to 1.0 indicating potential infection. The ELISA test detects the presence of antibodies in the blood, essentially categorizing normal from abnormal results.For lumbar puncture CSF tests, it’s a good idea to have someone else drive you home. What to bring: While you should leave valuables at home, it’s a good idea to have your ID, a list of medications you’re taking, as well as a notebook for note-taking.Talk to your insurer to find out what is covered. Cost and health insurance: Insurance plans can vary when it comes to testing and treatment for Lyme disease, with some companies being much more restrictive.Since some types of blood tests that might be drawn at the same time may require fasting beforehand, it’s worth checking with your healthcare provider. Food and drink: Generally speaking, there are no dietary restrictions before these tests.Those who need lumbar puncture will be asked to change into a gown. What to wear: Loose-fitting, comfortable clothes are fine for blood draws.Those getting lumbar puncture are prone on a bed, examination table, or operating table. Location: Samples of blood and CSF can be taken at a clinic or in the hospital.A lumbar puncture for CSF takes about 45 minutes for the procedure, then lying flat for an hour or two. Timing: A blood draw takes about five minutes.At this stage, the infection often has spread to the central nervous system, so CSF antibody or PCR tests can also help. Stage 3: The antibodies produced to fight off Lyme disease persist in the body long after infection, so two-tiered testing could still detect an advanced form of Lyme disease.An initial positive or inconclusive result (usually an ELISA test) will need to be repeated for confirmation (usually the Western blot test). At this point, the Centers for Disease Control and Prevention (CDC) recommends a two-stage testing process. Stage 2: During the “early disseminated” stage of Lyme disease, the infection has begun spreading to other bodily systems.Since it takes a couple of weeks for the body to develop antibodies, tests like ELISA are less likely to be accurate in this phase. Early stage: Within a month of exposure to Lyme disease, during its “localized” phase, testing isn’t generally necessary if Lyme-specific symptoms, such as the rash, are present.medical-conditions/sexual-and-reproductive-health/hiv-aids/diagnosis/western-blot-test. Updated CDC recommendation for serologic diagnosis of Lyme disease.cdc.gov/hiv/basics/hiv-testing/test-types.html Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Western blot: Technique, theory, and troubleshooting. aboutAPHL/publications/Documents/ID_HIV-1-1-WesternBlotBrief_62015.pdf Limitations for the use of HIV-1 western blot in plasma/serum.5/case-of-month-possible-false-positive-hiv-test/ Case of the month: Possible false positive HIV test.You can learn more about how we ensure our content is accurate and current by reading our editorial policy. Healthline has strict sourcing guidelines and relies on peer-reviewed studies, academic research institutions, and medical associations. Since it can take several weeks or months before antibodies are found in blood, the Western blot test may not always be reliable. If it causes a change in color, antibodies to a specific infection have been detected. The proteins are placed onto blotting paper that’s made from a material such as nitrocellulose.Īn enzyme is added to the paper. The Western blot uses a procedure called gel electrophoresis to identify and separate proteins by molecular weight and length. Antigens stimulate your immune system to push out antibodies in an effort to fight the disease. ![]() If you develop a viral, fungal, or bacterial infection, your body will produce proteins called antigens in response. ![]() The Western blot test looks for the antibodies against an infection, not the infection itself. If you get a positive result for Lyme disease or HIV after taking an enzyme-linked immunosorbent assay (ELISA) test, your doctor may recommend a Western blot test to you. It was invented in 1979 to identify ribosomal RNA binding proteins. The Western blot test is also known as a protein immunoblot test or immunoblotting. ![]()
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